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KMID : 0882419930450030353
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Korean Journal of Medicine
1993 Volume.45 No. 3 p.353 ~ p.360
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A Study about the Involvement of H-ras Oncogene in Acromegalic Patients
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엄승길
권이현/정윤석/안광진/이은직/김경래/이현철/허갑범/김태승
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Abstract
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ackground : Little is known about the mechanism of tumorigenesis in pituitary adenomas.
An important finding in somatotroph adenomas is that a somatic mutation may convert a G
protein, Gs(alpha) into a putative oncogene termed "gsp" via point mutations at two critcal
sites. The "ras" protooncogenes are structurally related to the G-protein family and are
involved in cell proliferation and differentiation. Although "ras" oncogene mutations have been
indentified in a wide variety of human neoplasm, only one case was reported as containting
single point mutation in a patient with invasive prolactionoma. In this report we used
oligonucleotide-specific hybridization to screen "ras" mutations in 13 acromegalic tumors.
Methods : Pituitary tissue samples were derived from a central portion of the paraffin
embedded pituitary tumor to minimize the possibility of contamination with normal tissue.
Genomic DNA was isolated and purified from tumor tissue and amplified by the standard
PCR method. Amplified DNAs from each of the region of H-"ras" genes (12/13 and 61) were
analyzed for potential "ras" mutations using oligonucleotide-specific hybridization as described
previously.
Results : Wild type radiolabelled oligoncleotides were hybridized to the amplified DNAs
from the patients' tumor and to the positive specimens. They were, however, easily striped
out at 68℃ by nonstringent washing procedures except control (wild type) specimens. All
radiolabelled mutant oligonucleotides could be easily striped out of 13 specimens except a
control mutant specimen by the same procedure.
Conclusion : We could not find any H-"ras" mutation that might not be frequently found
in acromegalic patients, and that "gsp" (Gsalpha mutation) or mutations in the PKA
system-related proteins might be the main oncogene in acromegalic patients. However
further efforts to find the other somatic mutations including K-"ras" and N-"ras" should be
given to these patients for more precise understanding of pathogenesis and for planning of
the better treatment.
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KEYWORD
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